Extreme Performance

All the power you need when you need it most

MICRO Models

  • Novel noise reduction algorithm implemented in an Altera™ FPGA
  • MICRO-PLUS model tuned for extreme small particle applications: extracellular vesicles, virus applications (flow virometry), protein aggregation, bacteria, nanoparticles
  • MICRO model tuned for the study of bacteria while still being suitable for a wide range of other applications (0.2µm to 20µm)
  • Unprecedented sensitivity and resolution from multiple light scatter and fluorescence detectors
  • Fast PCIe data acquisition electronics delivers up to 100k events per second
  • Up to 3 spatially separated lasers, 12 optical detectors, optional Autosampler
  • Patented light scatter size calibration module

Conventional flow cytometers on the market today may be able to resolve 200nm diameter latex beads by light scatter but they will fall close to the instrument’s sensitivity limit and latex is far more refractive (refractive index approximately 1.59) than biological particles (refractive index typically 1.40). For example, a 400nm biological particle may scatter about the same as a 200nm latex bead. Conventional flow cytometers are therefore not suitable for measuring light scattered by biological particles less than about 400nm diameter.

The new Micro-PLUS is capable of measuring particles which scatter 1000 times less than 200nm latex beads. The amount of scattered light falls rapidly with particle size so this exceptional performance is required for extreme small particle applications and opens up new areas of research.

Performance where it counts

Micro Flow Cytometer Chart

The ApogeeMix beads (Cat# 1493) are intended for instrument performance assessment. The beads range in size from 110nm to 1300nm and include silica beads with a refractive index similar to biological particles.

Don't just take our word for it...

Automation & Flexibility

Automation

  • Fully automated cleaning cycles
  • Fully automated start-up and shutdown
  • Autosampler option for labs where throughput and automation are a priority
  • Email alerts notify operator of status changes
  • Volumetric sample system gives absolute count for every sample

Flexibility

  • Fully upgradeable to a 3 laser system with 12 optical detectors plus Autosampler
  • Suitable for a wide range of applications (maximum particle size 100µm)

Microparticles & Microbiology

The Micro Flow Cytometer is in a class of its own for sub-micron biological particles, and leading the field in extracellular vesicle research.

Extracellular Vesicles
(Microparticles & Exosomes)

  • The Micro-PLUS model offers unprecedented sensitivity from two or three light scatter angle ranges
  • Microvesicles are set to provide the next generation of diagnostic assays
    • Blood-based diagnostic tests for cancers
    • Vascular biology & thrombosis research
    • Therapeutic drug delivery
  • Conventional flow cytometers rely on fluorescent probes to measure biological particles smaller than 400nm diameter, so data is poor when the fluorescence is dim and data inconsistent when the brightness of the fluorescent labels varies
  • The Micro Flow Cytometer's extreme light scatter performance allows particles' light scatter characteristics to be used to discriminate particles of interest
CD61-PE

CD61-PE (vertical axis) labelled platelet derived extracellular vesicles shown on a datagram (light scatter on X axis) with regions of interest left over from analysing test beads (both non-fluorescent silica and fluorescent latex). Note that all the CD61 positive vesicles scatter less than 300nm silica bead region (“10 300Si”) and that 300nm silica beads scatter less than the sensitivity limit of conventional flow cytometers.

Microbiology

  • Rapid detection of bacteria in wide variety of samples
  • Cell cycle analysis & genetics
  • Cell viability
  • Fluorescent proteins
  • Virology
Micro Flow Cytometer Chart 2

Lactobacillus and Propioni superimposed on polystyrene bead data demonstrating the importance of refractive index considerations. Most bacteria scatter a bit less than 500nm polystyrene beads. Many bacteria scatter less than 300nm polystyrene beads. The forward scatter detector on conventional flow cytometers is limited to particles which scatter more than 500nm beads so has insufficient sensitivity to be of use. Size & light scatter depend on growth conditions. Microbiology applications require the ability to resolve bacteria by light scatter, whatever the growth conditions, and by more than one light scatter detector.

Flow Virometry

The Micro-PLUS model can measure biological particles down to about 100nm diameter by light scatter. This opens up new applications in virology where both light scatter and fluorescence can be measured from the virions.

For further information see:

Josef Vlasak et al.
Use of flow cytometry for characterization of human cytomegalovirus vaccine particles.
Vaccine, March 2016. DOI:10.1016/j.vaccine.2016.03.067

Don't just take our word for it...

...performance where it counts

Platelets & Other Applications

Platelets

The Micro Flow Cytometer's high sensitivity is well suited to platelet applications, including:

  • platelet reactivity
  • platelet aggregates
  • circulating activated platelets
  • platelet-derived microparticles
  • calcium flux
  • bacterial contamination

Other Applications

The Micro Flow Cytometer is suitable for any analytical flow cytometry application where the particles of interest are less than 100µm, including:

  • Rapid detection of bacteria in wide variety of samples
  • Cell cycle analysis & genetics
  • Cell viability
  • Fluorescent proteins
  • Virology

Don't just take our word for it...

Don't just take our word for it...

Get In Touch
Follow:
Visit our Facebook page Follow us on Twitter Connect with us on LinkedIn
Share: